RESEARCH INTEREST
Molecular Biology and Cloning
Protein Expression and Bioanalytical Techniques
Cell Culture
Laboratory Operations and Lab Management
Soil Microbial Ecology
Metagenomics
Metabolomics
Bioinformatics
RESEARCH INTEREST
Molecular Biology and Cloning
Protein Expression and Bioanalytical Techniques
Cell Culture
Laboratory Operations and Lab Management
Soil Microbial Ecology
Metagenomics
Metabolomics
Bioinformatics
GRADUATE RESEARCH ASSISTANT (PhD):
Department of Crop and Soil Sciences
North Carolina State University- Raleigh, NC
Dissertation: The Turfgrass Root Microbiome: Responses to the Environment Stress and Mechanisms for Promoting Resilient Plants.
Advisor(s): Dr. Wei Shi (Soil Microbial Ecology Lab)
Independently performed molecular, microbiological, and biochemical assays involving environmental and biological samples while troubleshooting experimental workflows and optimizing protocols.
Performed DNA extraction from complex environmental matrices, including bulk soil, rhizosphere soil, root endosphere, and pure bacterial cultures, followed by amplicon sequencing, shotgun metagenomics, metabolomics, and bioinformatics analyses to investigate microbial functional responses to environmental stress.
Conducted ELISA-related protein quantification assays, fluorometric assays, Bradford protein assays, SDS-PAGE, gel electrophoresis, and Western blotting support workflows for protein analysis and characterization.
Executed molecular cloning workflows involving Gibson Assembly, bacterial transformation, restriction digestion, PCR validation, and Sanger sequencing for recombinant gene construct verification.
Performed recombinant protein expression in bacterial systems, followed by affinity chromatography-based protein purification and protein characterization analyses.
Conducted mammalian cell culture, transfection, and calcium signaling assays under aseptic laboratory conditions to evaluate gene expression and protein functionality.
Prepared reagents, buffers, media, biological samples, and plate-based assays while performing accurate pipetting, assay setup, and experimental processing under aseptic laboratory conditions.
Participated in assay development, optimization, troubleshooting, and validation for molecular cloning, protein expression, fluorometric assays, and microbiological analyses.
Isolated and characterized bacterial strains using biochemical and microbiological assays, including phosphate solubilization, ACC deaminase activity, siderophore production, nitrogen fixation, and IAA production.
Maintained accurate laboratory records, sample tracking, inventory organization, and experimental documentation while adhering to laboratory SOPs, safety protocols, and compliance requirements.
Compiled, analyzed, and visualized complex experimental datasets using Microsoft Excel, R, QIIME2, KBase, MetaboAnalyst, and other analytical software platforms while contributing to peer-reviewed publications and scientific conference presentations.
Collaborated with multidisciplinary research teams to complete multiple concurrent projects under strict timelines while ensuring data accuracy, reproducibility, and quality standards.
Trained and supervised new PhD students and summer undergraduate interns on XXXXXXX in the Soil Microbial Ecology lab.
GRADUATE RESEARCH ASSISTANT (MS):
Department of Soil, Water and Environment
University of Dhaka- Dhaka, Bangladesh
Dissertation: Role of Microbial Communities in Carbon Stock Distribution in Sundarbans Mangrove Forest in Bangladesh.
Supervisor: Dr. Zakia Parveen
Conducted interdisciplinary research on soil microbial ecology and biogeochemical processes, investigating microbial contributions to carbon sequestration and nutrient cycling in mangrove ecosystems.
Isolated, cultured, and characterized environmental microorganisms using microbiological, biochemical, and molecular techniques to evaluate microbial diversity and functional potential.
Prepared and optimized culture media, reagents, buffers, and biological samples while performing aseptic laboratory procedures, precision pipetting, and plate-based experimental assays.
Maintained comprehensive laboratory documentation, sample traceability, inventory management systems, and quality-controlled experimental records in compliance with laboratory SOPs and safety regulations.
Evaluated the influence of microbial communities on organic carbon partitioning among cold-water soluble, hot-water soluble, labile, moderately labile, and recalcitrant carbon pools in Sundarbans mangrove soils.
Collaborated with governmental research institutes, environmental agencies, and academic researchers to support experimental design, field sampling campaigns, laboratory analyses, and statistical data interpretation.
Performed physicochemical and microbiological analyses of soil, water, and plant samples, including nutrient quantification (NO₃⁻, PO₄³⁻), total organic carbon (TOC), and heavy metal determination (Pb, Cd, Cr, Ni, Zn, Cu, Mn, Fe) using standard analytical methodologies.
UNDERGRADUATE RESEARCH
Department of Soil, Water and Environment
University of Dhaka- Dhaka, Bangladesh
Thesis Title: Ship Breaking Industry in Bangladesh: Environmental Pollution and Associated Ecological Risks.
SUPERVISOR: Dr. A.S.M. Mohiuddin