TECHNICAL SKILLS:

• Molecular Biology and Cloning: DNA Extraction, PCR, qPCR, Gibson Assembly, Molecular Cloning, Restriction Digestion, Clone Screening, Recombinant DNA Workflows, Gene Expression Analysis, Next-Generation Sequencing Library Preparation (Amplicon and Shotgun)

• Protein Expression and Bioanalytical Techniques: Recombinant Protein Expression (Prokaryotic & Eukaryotic Systems), Protein Purification (Affinity Chromatography), SDS-PAGE, Gel Electrophoresis, Western Blotting, Bradford Protein Assay, ELISA, Fluorometric Assays, Immunoassays

• Cell Culture: Mammalian and Bacterial Cell Culture, Cell Passaging, Media Preparation, Transfection, Calcium Signaling Assays, Aseptic Techniques

• Laboratory Operations and Lab Management: Assay Development & Troubleshooting, Reagent & Buffer Preparation, SOP Compliance, Laboratory Documentation, Hazardous Chemical Handling, Statistical Analysis